Premium
In vitro effects of sEng and TGF‐β on human umbilical vein endothelial cells and trophoblasts
Author(s) -
Wang Ketao,
Lu Caihua,
Liu Yanpo,
Tao Ye
Publication year - 2018
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13643
Subject(s) - umbilical vein , andrology , medicine , flow cytometry , enos , apoptosis , in vitro , microbiology and biotechnology , immunology , biology , nitric oxide , biochemistry , nitric oxide synthase
Aim The aim of this study was to evaluate the in vitro effects of sEng and TGF‐β on human umbilical vein endothelial cells (HUVECs) and trophoblasts. Methods HUVECs and trophoblasts were treated with 1, 5, 10, 15, 30, 50, 80 and 100 μg/L sEng, TGF‐β or (sEng + TGF‐β) for 6, 12, 24, 36, 48 and 72 h, respectively. sEng, TGF‐β and NO levels in culture media of HUVECs were measured by ELISA. Survival rates of HUVECs and trophoblasts were detected by 3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyltetrazolium bromide (MTT) assay. Apoptotic rate and cell cycle distribution were detected by flow cytometry. eNOS, MMP‐2 and MMP‐9 expressions were detected. Results The survival rate of 100 μg/L of sEng‐treated HUVECs was significantly lower than those of other groups at the same time point ( P < 0.05). The survival rate of the TGF‐β group was significantly lower than that of the sEng group after treatment at each concentration for 36 and 48 h ( P < 0.05). NO content significantly decreased with increasing sEng concentration ( r = 0.89, P < 0.05) and with extended time at identical sEng concentration ( r = 0.78, P < 0.05). The number of membrane‐penetrating trophoblasts in the control group significantly surpassed that of sEng group ( P < 0.05). The number of expressions in the TGF‐β group significantly exceeded that of control group ( P < 0.05), being significantly different from that of sEng group ( P < 0.05). MMP‐2 and MMP‐9 expressions in sEng group were significantly lower than those of the control group ( P < 0.05), and such expressions of the TGF‐β group significantly surpassed those of other groups ( P < 0.05). Conclusion sEng and TGF‐β were closely associated with endothelial cell injury and abnormalities of trophoblast proliferation and infiltration in vitro .