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Pretreatment with magnesium sulfate attenuates white matter damage by preventing cell death of developing oligodendrocytes
Author(s) -
Seyama Takahiro,
Kamei Yoshimasa,
Iriyama Takayuki,
Imada Shinya,
Ichinose Mari,
Toshimitsu Masatake,
Fujii Tomoyuki,
Asou Hiroaki
Publication year - 2018
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13568
Subject(s) - periventricular leukomalacia , medicine , white matter , microglia , lactate dehydrogenase , myelin basic protein , tunel assay , anesthesia , endocrinology , andrology , pharmacology , myelin , biology , biochemistry , gestational age , inflammation , pregnancy , central nervous system , immunohistochemistry , genetics , radiology , magnetic resonance imaging , enzyme
Aim Antenatal maternal administration of magnesium sulfate (MgSO 4 ) reduces cerebral palsy in preterm infants. However, it remains controversial as to whether it also reduces occurrence of white matter damage, or periventricular leukomalacia. We assessed the effect of MgSO 4 against white matter damage induced by hypoxic‐ischemic insult using a neonatal rat model and culture of premyelinating oligodendrocytes (pre‐OL). Methods Rat pups at postnatal day (P) 6 were administered either MgSO 4 or vehicle intraperitoneally before hypoxic‐ischemic insult (unilateral ligation of the carotid artery followed by 6% oxygen for 1 h). The population of oligodendrocyte (OL) markers and CD‐68‐positive microglia at P11, and TdT‐mediated biotin‐16‐dUTP nick‐end labeling (TUNEL)‐positive cells at P8 were evaluated in pericallosal white matter. Primary cultures of mouse pre‐OL were subjected to oxygen glucose deprivation condition, and the lactate dehydrogenase release from culture cells was evaluated to assess cell viability. Results Pretreatment with MgSO 4 attenuated the loss of OL markers, such as myelin basic protein and Olig2, in ipsilateral pericallosal white matter and decreased the number of CD‐68‐positive microglia and TUNEL‐positive cells in vivo . Pretreatment with MgSO 4 also inhibited lactate dehydrogenase release from pre‐OL induced by oxygen glucose deprivation in vitro . Conclusion Pretreatment with MgSO 4 attenuates white matter damage by preventing cell death of pre‐OL.