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Endothelin‐1‐induced expression of α‐smooth muscle actin in human myometrial fibroblasts
Author(s) -
Duan Zhaoning,
Jia Ying,
Zhang Jing,
Long Li,
Tang Liangdan
Publication year - 2018
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13539
Subject(s) - myometrium , sma* , western blot , endothelin 1 , messenger rna , immunohistochemistry , microbiology and biotechnology , rna , andrology , in vitro , gene expression , medicine , smooth muscle , endocrinology , fibroblast , biology , gene , receptor , uterus , biochemistry , mathematics , combinatorics
Aim We aimed to investigate the potential role of endothelin‐1 ( ET ‐1) in the regulation of the expression of α‐smooth muscle actin (α‐ SMA ) in human myometrial fibroblasts. Methods Primary myometrial fibroblasts were obtained from myometrium and were identified by immunocytochemical staining. Then, 1 × 10 7 cells were treated with ET ‐1 at a concentration of 0.1, 1.0, 10.0, or 100.0 n M for 24 h. To investigate the time course effects of ET ‐1 on the growth of fibroblasts, 1 × 10 7 cells were treated with 10.0 n M ET ‐1 for 6, 12, 24, and 48 h. Real‐time quantitative PCR (qPCR) and W estern blot analysis were used to determine the expression of α‐ SMA m RNA and protein, respectively. Results Human myometrial fibroblasts were identified by immunohistochemistry. Compared with the control group, the expression levels of α‐ SMA m RNA and protein were identified in cells treated with ET ‐1 at a concentration of 0.1, 1.0, 10.0, or 100.0 n M for 24 h ( P < 0.05). ET ‐1 treatment affected the expression of α‐ SMA m RNA and protein in a dose‐dependent manner ( P < 0.05). The induction of α‐ SMA m RNA and protein expression increased from 6 to 48 h. Conclusion The results show that ET ‐1 induces the expression of α‐ SMA in human myometrium in vitro.