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Long non‐coding RNA LINC00261 inhibits cell growth and migration in endometriosis
Author(s) -
Sha Lixiao,
Huang Lingxiao,
Luo Xishao,
Bao Jiaping,
Gao Lijun,
Pan Qionghui,
Guo Min,
Zheng Feiyun,
Wang Hanchu
Publication year - 2017
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13427
Subject(s) - cell growth , flow cytometry , apoptosis , cell , cell migration , microbiology and biotechnology , endometriosis , cell culture , biology , cancer research , medicine , pathology , biochemistry , genetics
Aim A previous study reported that LINC00261 is significantly downregulated in human ectopic endometrial tissues. The present study aimed to explore whether LINC00261 is functional in endometriosis cell proliferation, apoptosis and migration. Methods By transfecting human endometriosis cell line CRL‐7566 with plasmids containing LINC00261, we successfully established the cell CRL‐7566/LINC00261 with a high LINC00261 expression level. Cell‐counting kit‐8 and colony formation assays were conducted to evaluate the effect of LINC00261 on cell proliferation, and flow cytometry analysis and transwell migration assay were conducted to evaluate its effect on cell apoptosis and cell migration, respectively. Results Cell‐counting kit‐8 and colony formation assays both indicated that LINC00261 could inhibit cell proliferation in CRL‐7566. Flow cytometry analysis confirmed that LINC00261 mediated inhibition of cell proliferation, which might be a consequence of inducting apoptosis. Furthermore, transwell migration assay indicated that LINC00261 could inhibit cell migration in endometriosis. Conclusion LncRNA LINC00261 is capable of inhibiting cell growth and migration in endometriosis.