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Antioxidant properties of coenzyme Q10‐pretreated mouse pre‐antral follicles derived from vitrified ovaries
Author(s) -
Hosseinzadeh Elmira,
Zavareh Saeed,
Lashkarbolouki Taghi
Publication year - 2017
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13173
Subject(s) - coenzyme q10 , medicine , antral follicle , antioxidant , andrology , ovary , antrum , biochemistry , biology , stomach
Aim This study evaluated the antioxidant status of pre‐antral follicles derived from vitrified ovaries pretreated with coenzyme Q10 (CoQ10). Methods Mouse pre‐antral follicles derived from fresh and vitrified warmed ovarian tissue were cultured with or without CoQ10 (50 μmol/L). Follicular growth, total antioxidant capacity (TAC), malondialdehyde (MDA) level, and superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activity during cultivation were assessed. Results The growth rate of the fresh pre‐antral follicles was higher compared with the vitrified groups, especially in the CoQ10‐treated than non‐treated groups. MDA increased while TAC decreased at 96 h of the cultivation period. TAC was higher while MDA was lower in the fresh pre‐antral follicles than in the vitrified groups. These rates were higher in the CoQ10‐treated than non‐treated groups. The vitrified and fresh CoQ10‐pretreated groups had significantly higher SOD, GPX, and CAT activity compared with the CoQ10 non‐treated groups. Conclusion CoQ10‐supplemented maturation medium can increase antioxidant enzyme activity and decrease lipid peroxidation in cultured pre‐antral follicles derived from fresh and vitrified mouse ovaries.