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Influence of carbon dioxide pneumoperitoneum on the growth hormone–insulin‐like growth factor I axis in mid‐ and late‐pregnancy rats
Author(s) -
Sun Bingchun,
Fu Aizhen,
Wang Rong,
Zhang Ying
Publication year - 2015
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12734
Subject(s) - pneumoperitoneum , medicine , pregnancy , insulin like growth factor , endocrinology , hormone , fetus , growth factor , surgery , biology , genetics , receptor , laparoscopy
Aim We investigated the influence of carbon dioxide (CO 2 ) pneumoperitoneum on the growth hormone(GH)–insulin‐like growth factor I (IGF‐I) axis in mid‐ and late‐pregnancy Sprague–Dawley rats. Methods A total of 48 mid‐pregnancy rats were randomly assigned to one of three groups: anesthesia only (control group); 1‐h CO 2 pneumoperitoneum; or 2‐h CO 2 pneumoperitoneum. Blood samples were collected immediately after the procedure or in late pregnancy. Maternal concentration of serum GH and IGF‐I was measured on enzyme‐linked immunosorbent assay and compared between different groups. Results Under the same CO 2 pneumoperitoneum pressure, serum GH and IGF‐I concentration in the 2‐h pneumoperitoneum group were significantly lower than those of the 1‐h pneumoperitoneum group or the control group in both mid‐ and late pregnancy ( P < 0.05), but there was no significant difference between the 1‐h pneumoperitoneum group and the control group ( P > 0.05). Serum GH and IGF‐I concentrations were positively correlated in pregnant rats ( R 2 = 0.3434, P < 0.05). Conclusions Under the same CO 2 pneumoperitoneum pressure, exposure duration was correlated with effect on maternal GH–IGF‐I axis in mid‐ and late‐pregnancy rats. Two h of exposure inhibited the GH–IGF‐I axis in both mid‐ and late pregnancy, and therefore may restrict development of the placenta and fetus.