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OCT ‐4 and DAZL expression in precancerous lesions of the human uterine cervix
Author(s) -
Stefanidis Konstantinos,
Pergialiotis Vasileios,
Christakis Dimitrios,
Patta Jessica,
Stefanidi Diamanto,
Loutradis Dimitrios
Publication year - 2015
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12640
Subject(s) - cervical intraepithelial neoplasia , medicine , cervical cancer , downregulation and upregulation , andrology , intraepithelial neoplasia , hpv infection , immunohistochemistry , cervix , carcinogenesis , pathology , cancer research , biology , cancer , prostate , gene , biochemistry
Aim To determine whether octamer‐binding transcription factor 4 ( OCT ‐4 ) and deleted in azoospermia like ( DAZL ) are expressed among cells with human papilloma virus ( HPV ) infection and cervical intraepithelial neoplasia ( CIN ) lesions and quantify their relative expression when compared with normal cervical cultures. Methods Cervical cells derived from normal cell cultures, HPV lesions and CIN lesions were cultured in Dulbecco's modified Eagle's medium supplemented with 20% amniotic fluid and 5 ng/mL basic fibroblast growth factor at 37°C and humidified 10% CO 2 in air. Real‐time polymerase chain reaction ( PCR ) was carried out using G6PD as a reference. We used REST for statistical analysis of real‐time PCR . Results Whereas DAZL was not expressed either in normal cultures or HPV and CIN lesions, OCT ‐4 was expressed in all examined cell lines. Moreover its relative expression was significantly upregulated among cultures of HPV ‐infected cells ( RE , 11.003; 95% CI : 0.054–36 704.527, P = 0.042), an observation that was also close to statistical significance among cultures of CIN lesions ( P = 0.066). Conclusion The relative expression of OCT ‐4 is upregulated during the early, preinvasive stages of cervical carcinogenesis. Future studies should investigate its potential as a screening marker and as a possible target of therapy.