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Analysis of gene expression and preliminary study of methylation about 11β‐ HSD2 gene in placentas of C hinese pre‐eclampsia patients of Han ethnicity
Author(s) -
Hu Wensheng,
Wang Hanzhi,
Huang Hefeng
Publication year - 2015
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12555
Subject(s) - methylation , microbiology and biotechnology , placenta , polymerase chain reaction , bisulfite sequencing , immunohistochemistry , andrology , gene expression , promoter , bisulfite , trophoblast , messenger rna , gene , staining , epigenetics , western blot , medicine , biology , dna methylation , pathology , fetus , genetics , pregnancy
Aims The aim of this study was to determine the promoter methylation status of type 2 isoform of 11β‐hydroxysteroid dehydrogenase (11β‐ HSD2 ) and its regulatory correlation with 11β‐ HSD2 gene expression in placentas of pre‐eclampsia ( PE ) patients of C hinese H an ethnicity. Material and Methods The pathological features of placental tissues were studied using hematoxylin–eosin staining and immunohistochemical staining. The 11β‐ HSD2 mRNA and protein expressions were detected by real‐time polymerase chain reaction and W estern blotting. The methylation of the 11β‐ HSD2 promoter sequence was examined by bisulfite sequencing polymerase chain reaction. Results Trophoblast hyperplasia and discontinuous syncytial layer were observed in the PE group, and the 11β‐ HSD2 was distributed irregularly and its immunoreactivity was weakened distinctly. The expressions of 11β‐ HSD2 mRNA and protein decreased significantly in the PE group compared with the control group. Unexpectedly, almost no 11β‐ HSD2 methylation was detected in PE placental tissue (two fragments, 0.6% vs 0%) or normal placental tissue (1% vs 0.6%). No significant difference in 11β‐ HSD2 promoter methylation was found between the two groups. Conclusions The 11β‐ HSD2 expression decreased in PE women of C hinese Han ethnicity, but was not interrelated with the promoter methylation status.