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Expression of placenta growth factor, soluble fms‐like tyrosine kinase‐1, metal‐responsive transcription factor‐1, heme oxygenase 1 and hypoxia inducible factor‐1α mRNAs in pre‐eclampsia placenta and the effect of pre‐eclampsia sera on their expression of choriocarcinoma cells
Author(s) -
Maebayashi Asanuma Aki,
Yamamoto Tatsuo,
Azuma Hiromitu,
Kato Erina,
Yamamoto Noriko,
Murase Takayuki,
Chishima Fumihisa,
Suzuki Manami
Publication year - 2014
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12462
Subject(s) - placenta , trophoblast , placental growth factor , messenger rna , heme oxygenase , eclampsia , hypoxia inducible factors , reverse transcription polymerase chain reaction , medicine , andrology , endocrinology , heme , microbiology and biotechnology , vascular endothelial growth factor , fetus , chemistry , biology , pregnancy , gene , enzyme , biochemistry , vegf receptors , genetics
Aim We studied the effect of pre‐eclampsia sera on the expression of placenta growth factor ( PlGF ), soluble fms‐like tyrosine kinase‐1 (s F lt‐1), metal‐responsive transcription factor‐1 ( MTF ‐1), heme oxygenase 1 ( HO ‐1) and hypoxia inducible factor‐1α ( HIF ‐1α) mRNAs in JEG ‐3 cells (trophoblast‐derived cells) and placenta from pre‐eclampsia patients to investigate pre‐eclampsia pathophysiology. Material and Methods Placenta and serum samples were taken from pre‐eclampsia and normal pregnancy patients. JEG ‐3 cells were cultured with pre‐eclampsia and normal pregnant sera in 24‐well tissue culture plates. RNA was purified from placental trophoblast cells and JEG ‐3 cells 24 h after incubation. The expression of mRNA was measured using real‐time polymerase chain reaction. Results The expression of s F lt‐1 mRNA increased, and that of PlGF and HO ‐1 mRNA decreased in JEG ‐3 cells after incubation with pre‐eclampsia sera. The expression of PlGF mRNA decreased, and that of s F lt‐ 1mRNA increased in pre‐eclampsia placenta. The expression of MTF ‐1 and HO ‐1 mRNA decreased. A correlation was found between PlGF mRNA expression and the expression of MTF ‐1 and HIF ‐1α mRNA . A correlation between s F lt‐1 and HIF ‐1α mRNA expression was also found. Conclusion Changes in PlGF mRNA expression in pre‐eclampsia placenta may relate to serum factors and the expression of MTF ‐1 and HIF ‐α mRNA . Changes in s F lt‐1 mRNA expression may relate to serum factors and the expression of HIF ‐α mRNA . We suggest that serum factors play a role in PlGF and s F lt‐1 expression in pre‐eclampsia placenta.