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Impact of first trimester fasting glycemic levels on expression of proteoglycans in pregnancy
Author(s) -
Capobianco Giampiero,
De Muro Pierina,
Lepedda Antonio Junior,
Dessole Margherita,
Ambrosini Guido,
Cherchi Pier Luigi,
Formato Marilena
Publication year - 2014
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12411
Subject(s) - medicine , urine , endocrinology , pregnancy , glycemic , excretion , urinary system , gestational diabetes , diabetes mellitus , gestation , biology , genetics
Aim The aim of this study was to assess the influence of glucose metabolism on the expression of glycosaminoglycans ( GAG s) and proteoglycans ( PG s) in pregnant women. Material and Methods Seventy‐six women in the first trimester of pregnancy (10–13 weeks) attending the Gynecologic and Obstetric Clinic, U niversity of S assari, were enrolled and gave early morning urine samples. Groups I , II and III included women with serum glucose values of 65–89 mg/dL, 90–99 mg/dL and 100–125 mg/dL, respectively. Urine GAG s/ PG s distribution was determined by electrophoresis on cellulose acetate strips. Urinary N ‐Acetyl‐β‐glucosaminidase was estimated kinetically. Results Analysis of urinary GAG s/ PG s electrophoretic profiles showed a significant increase in heparan sulfate ( HS ) excretion ( P = 0.017) as well as a reduced chondroitin sulfate ( CS ) excretion ( P = 0.048) in the group II pregnant women compared with the group I , and higher values of the HS / CS ratio in groups II and III compared to group I . Furthermore, we observed a positive correlation among fasting blood glucose levels and the relative content of HS , the HS / CS and urinary trypsin inhibitor/ CS ratios, and the N ‐Acetyl‐β‐glucosaminidase levels. Conclusions The assessment of risk factors for gestational diabetes mellitus should also take into account fasting blood glucose values of 90–99 mg/dL, as the findings of our study indicated an alteration in the metabolism of GAG s during the early stages of pregnancy.