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Possible association between stem‐like hallmark and radioresistance in human cervical carcinoma cells
Author(s) -
Kumazawa Shoko,
Kajiyama Hiroaki,
Umezu Tomokazu,
Mizuno Mika,
Suzuki Shiro,
Yamamoto Eiko,
Mitsui Hiroko,
Sekiya Ryuichiro,
Shibata Kiyosumi,
Kikkawa Fumitaka
Publication year - 2014
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12357
Subject(s) - radioresistance , sox2 , side population , apoptosis , hela , stem cell , flow cytometry , cxcr4 , cancer stem cell , cancer research , population , microbiology and biotechnology , cell culture , cell , biology , medicine , immunology , gene , biochemistry , genetics , chemokine , immune system , environmental health , transcription factor
Aim We aimed to investigate the possibility of an association between a stem‐like hallmark and radiotherapeutic sensitivity in human cervical carcinoma cells. Material and Methods Side‐population ( SP ) cells and non‐ SP ( NSP ) cells in HeLa cells were isolated using flow cytometry and Hoechst 33342 efflux. We performed W estern blot analysis to evaluate the expression of stem cell markers ( CXCR4 , O ct3/4, CD133 , and SOX2 ) and apoptosis markers after irradiation. In addition, SP and NSP cells were injected into nude mice and we assessed subcutaneous tumor formation. To examine tolerance of irradiation, colony formation and apoptosis change were confirmed in the SP and NSP cells. Results SP cells showed a higher expression of CXCR4 , O ct3/4, CD133 , and SOX2 than NSP cells. The colony size of SP cells cultured on non‐coated dishes was larger than that of NSP cells, and NSP cells were easily induced to undergo apoptosis. SP cells tended to form spheroids and showed a higher level of tumorigenicity compared with NSP cells. In addition, nude mice inoculated with SP cells showed greater tumor growth compared with NSP cells. SP cells showed a higher tumorigenicity and lower apoptotic potential, leading to enhanced radiotolerance. Conclusion Tumor SP cells showed higher‐level stem‐cell‐like characters and radioresistance than NSP cells. SP cells may be useful for new therapeutic approaches for radiation‐resistant cervical cancer.