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Mutational analysis of FOXL2 p. C134W and expression of bone morphogenetic protein 2 in J apanese patients with granulosa cell tumor of ovary
Author(s) -
Oseto Kumiko,
Suzumori Nobuhiro,
Nishikawa Ryutaro,
Nishikawa Hiroshi,
Arakawa Atsushi,
Ozaki Yasuhiko,
Asai Hidekazu,
Kawai Michiyasu,
Mizuno Kimio,
Takahashi Satoru,
Shirai Tomoyuki,
YamadaNamikawa Chisato,
Nakanishi Makoto,
Kajiyama Hiroaki,
Kikkawa Fumitaka,
SugiuraOgasawara Mayumi
Publication year - 2014
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12324
Subject(s) - immunostaining , ovary , immunohistochemistry , bone morphogenetic protein 2 , mutation , bone morphogenetic protein , follistatin , ovarian tumor , medicine , endocrinology , biology , cancer research , pathology , ovarian cancer , cancer , genetics , gene , in vitro
Aim To assess whether FOXL2 p. C134W mutation may play a role in the development of human ovarian tumors in the Japanese, we investigated the FOXL2 codon 134 mutation and protein expression of inhibin‐α, bone morphogenetic protein 2 ( BMP2 ) and follistatin ( FST ) in J apanese patients with granulosa cell tumor ( GCT ) of the ovary and other ovarian tumors. Methods We analyzed 114 tumor tissues from ovarian tumors, including 44 adult‐type and two juvenile‐type GCT of the ovary and 68 ovarian tumors by DNA sequencing. Immunohistochemistry was also performed in the adult and juvenile GCT tissues by immunostaining inhibin‐α, BMP2 and FST . Results We found the FOXL2 p. C134W mutation in 27 out of 44 (61.4%) adult‐type GCT of the ovary, but none in other ovarian tumors. Histologically, all of the adult‐type GCT sections were positive for inhibin‐α, and the expression of BMP2 and FST was detected in 14 of 44 (31.8%) and zero of 47 (0%), respectively. No significant differences regarding the diagnosed age, preoperative serum carbohydrate antigen 125 levels, or BMP2 immunopositivity between the FOXL2 p. C134W mutation‐positive and mutation‐negative were found in the adult‐type GCT patients. Conclusion Our findings suggest that FOXL2 p. C134W mutation‐positive adult‐type GCT of the ovary may not be common in the Japanese as compared to the previous data.