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Study for the improvement of umbilical cord blood sampling using a new trial apparatus
Author(s) -
Masaoka Naoki,
Morooka Masako,
Nakajima Yoshiyuki,
Ogata Hajime,
Kodo Hideki,
Kato Shunichi
Publication year - 2014
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.12179
Subject(s) - medicine , umbilical cord , blood sampling , sampling (signal processing) , transplantation , cord blood , gestational age , surgery , andrology , pregnancy , immunology , filter (signal processing) , biology , computer science , computer vision , genetics
Aim The aim of this study was to evaluate the usefulness of the trial umbilical cord blood sampling bag for unrelated cord blood transplantation. Material and Methods Data were obtained from 100 vaginal deliveries. In 50 cases, umbilical cord blood ( UCB ) was taken with the traditional K awasumi type UCB sampling bag. In another 50 cases, UCB were taken with trial UCB sampling bag offered by NIPRO C o. We compared the sampling volume between the two groups. Furthermore, 10 cases in each group were matched by sampling volume; we examined the quality of UCB on the number and concentration of nucleated cells, mononuclear cells, CD 34+ cells and colony‐forming unit granulocyte macrophage and the numbers tested positive for bacteria. Results Whereas there were no significant differences in gestational weeks at sampling, the ratio of primipara women to multipara women, maternal age, and neonatal weight between the two groups, the sampling UCB volumes with the trial sampling bag were significantly higher than those with traditional sampling bags ( P < 0.05). In addition, this phenomenon was more significant in the latter part of the study period ( P < 0.05). On the other hand, there were no significant differences in the quality of UCB between the two groups. Conclusion Once clinicians have become accustomed to the trial UBC sampling bag, this method might be a useful method for collecting UCB for unrelated cord blood transplantation.