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Basic Principles of Cryobiology
Author(s) -
Bank Harvey L.,
Brockbank Kelvin G.M.
Publication year - 1987
Publication title -
journal of cardiac surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 58
eISSN - 1540-8191
pISSN - 0886-0440
DOI - 10.1111/jocs.1987.2.1s.137
Subject(s) - cryobiology , medicine , cryopreservation , microbiology and biotechnology , biology , embryo
The ultimate aim of most cryopreservation procedures is the retention of the structural and functional integrity of the frozen cells or tissue. Designing strategies for achieving high “viability” of the frozen tissue requires an appreciation of the complex physical‐chemical events which occur during freezing and thawing, as well as the importance of other processing steps including tissue procurement and post‐thaw processing of the tissue, all of which can adversely affect the tissue. Here we have provided a general overview of cryobiology with special emphasis on techniques for organ preservation.