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miR‐6718‐5p and miR‐4329 can be used as potential biomarkers for acute myocardial infarction
Author(s) -
Chen Shaoyuan,
Fang Hongcheng,
Liu Rongzhi,
Fang Yeqing,
Wu Zhenyuan,
Xie Peiyi
Publication year - 2021
Publication title -
journal of cardiac surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 58
eISSN - 1540-8191
pISSN - 0886-0440
DOI - 10.1111/jocs.15868
Subject(s) - medicine , microrna , myocardial infarction , microvesicles , exosome , reverse transcriptase , polymerase chain reaction , real time polymerase chain reaction , downregulation and upregulation , bioinformatics , cardiology , gene , genetics , biology
Background The prevention and prognosis of the onset or recurrence of acute myocardial infarction (AMI) is a difficult problem in contemporary research. Methods In this study, peripheral blood samples were collected from seven patients with AMI and nine healthy adults, and exosome microRNAs (miRNAs) were extracted. The miRNA differential expression profiles of serum exosomes in patients with AMI were obtained by using the next‐generation sequencing technology combined with bioinformatics analysis. Reverse‐transcriptase polymerase chain reaction (RT‐PCR) was used to verify the primary screening of differential exosome miRNAs to reveal the possible mechanism of their action on AMI. Results Compared with healthy individuals, 544 miRNAs were upregulated and 518 miRNAs were downregulated in AMI patients preoperatively. Among these miRNAs, we selected miR‐6718 and miR‐4329 for qPCR verification. The expression of miR6718 and miR‐4329 in patients with myocardial infarction was significantly lower than that in normal controls.