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Predicting miRNA‐lncRNA‐mRNA network in ultraviolet A‐induced human skin photoaging
Author(s) -
Lin Yao,
Lin Mengbi,
Liu Yufang,
Zhang Jie,
Lai Wei,
Xu Qingfang,
Zheng Yue
Publication year - 2021
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.13760
Subject(s) - photoaging , microrna , biology , signal transduction , gene expression , kegg , messenger rna , long non coding rna , human skin , microbiology and biotechnology , rna , computational biology , gene , transcriptome , genetics
Background/Objective Recent researches had reported that microRNAs (miRNAs) played a role in skin photoaging. Our previous study found that long noncoding RNA (lncRNA) expression was changed in the UVA‐irradiated skin fibroblasts, but the regulating network of noncoding RNA in UV‐induced skin changes has not been elucidated well. Here, we investigated the interactions of miRNA‐lncRNA‐mRNAs in skin photoaging mechanisms. Methods Human dermal fibroblasts (HDFs) were irradiated with UVA at 10 J/cm 2 once a day lasting for 14 days. miRNA expression profiles were detected by high‐throughput sequencing. miRNAs changed significantly were identified by qRT‐PCR. Functional annotation analysis and pathway enrichment were carried out using Gene Ontology and KEGG, and predicted miRNA‐lncRNA‐mRNA interactions were performed via bioinformatic analysis. Results 34 differentially expressed miRNAs (>1.5‐fold changes, P < .05) after UVA irradiation were identified to interact with distinct lncRNAs. miRNA‐lncRNA‐mRNA network prediction and regulatory role analysis showed that the gene expression of cellular process, cell part, and binding was mainly coordinated in UVA‐irradiated fibroblasts. miRNA‐lncRNA‐mRNA‐signal transduction pathway analysis showed that TNF signaling pathway, thyroid hormone signaling pathway, and lysosome were mainly affected after UVA irradiation. Conclusion miRNA‐lncRNA‐mRNA network played a critical part in skin photoaging. Our research provided novel insights into the repeated UVA‐induced skin damage in noncoding RNA regulatory field and might help to further understand the delicate interplay of gene regulation at the noncoding RNA level in photoaged skin and UV‐induced skin cancers in future researching and provide novel insights into the repeated UVA‐damaging pathology and potential targets for preventing human skin photoaging.