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The anti‐inflammatory and anti‐apoptotic effects of advanced anti‐inflammation composition (AAIC) in heat shock‐induced human HaCaT keratinocytes
Author(s) -
Woo YongKyu,
Park Jungha,
Ryu JinHyeob,
Cho HyunJeong
Publication year - 2020
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.13257
Subject(s) - hacat , keratinocyte , apoptosis , heat shock protein , lipopolysaccharide , inflammation , chemistry , viability assay , western blot , medicine , immunology , biochemistry , in vitro , gene
Background The development of natural cosmetic materials without side effects to protect skin from heat shock is necessary. We recently reported that advanced cooling composition (ACC) has anti‐inflammatory effect in RAW 264.7 cells stimulated with lipopolysaccharide (LPS) and strong anti‐microbial effect against Pseudomonas aeruginosa , Staphylococcus aureus , MRSA (Methicillin‐resistant Staphylococcus aureus ), Candida albicans , and Streptococcus mutans . Aims To further investigate whether advanced anti‐inflammation composition (AAIC), newly developed from existing ACC has beneficial effects in heat shock‐induced immortalized human keratinocytes (HaCaT cells), HaCaT cells were pretreated with AAIC before heat shock treatment. Methods Cell viability for heat shock treatment and different concentrations of AAIC in HaCaT cells were assessed by MTT assay. Anti‐oxidative activity of AAIC was measured using the DPPH assay. The protein expression in heat shock‐induced HaCaT cells treated with AAIC was evaluated by immunofluorescence staining and western blot analysis. Results AAIC, which is effective at 100 µg/mL concentration, was nontoxic in HaCaT cells and had an anti‐oxidative effect demonstrated by scavenging DPPH free radicals. AAIC treatment significantly attenuated the aberrant levels of pro‐inflammatory and pro‐apoptotic signaling molecules in heat shock‐induced HaCaT cells compared with control cells. Conclusion AAIC potentially includes effective anti‐oxidative activity, anti‐inflammatory, and anti‐apoptotic properties against heat shock‐induced keratinocytes, suggesting that it can be provided as a raw material for imparting skin health.