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Effect of alerce ( Fitzroya cupressoides) cell culture extract on wound healing repair in a human keratinocyte cell line
Author(s) -
Carvajal Felipe,
Duran Catherine,
Aquea Felipe
Publication year - 2020
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.13137
Subject(s) - hacat , wound healing , cell culture , keratinocyte , context (archaeology) , viability assay , cytotoxicity , biology , in vitro , pharmacology , immunology , biochemistry , paleontology , genetics
Background Fitzroya cupressoides, commonly known as alerce, is an endemic conifer unique to southern South America. Alerce wood is renowned for its durability and resistance to biological degradation due to the presence of a particular class of secondary metabolite. Alerce extracts have been used in traditional medicine for different skin lesion treatments. Aims To develop a cell culture system to produce alerce extract and evaluate its cytotoxicity and effects on in vitro wound healing. Methods Cell cultures and aqueous extracts were prepared from alerce needles. Cytotoxicity was evaluated in keratinocytes (HaCaT line) and melanocites (C32 line) using the XTT assay. Wound healing was assayed with the scratch test in HaCaT cells, using mitomycin C to evaluate the role of cell division in the wound closure. Results Alerce cell culture extract has a significant effect on wound healing at different concentrations. No positive effects on the viability of normal and cancerous skin cells were observed. These results suggest that alerce extracts stimulate cell division in human skin epidermal cells in the context of wound repair. Conclusions Bioactive compounds extracted from alerce cell cultures show promise as ingredients in dermocosmetic formulations, but further clinical studies are required to support these findings at the tissue level.