Premium
Whitening effects of cosmetic formulation in the vascular component of skin pigmentation
Author(s) -
Pereira Andréia Feital Costa,
Igarashi Mamy Honda,
Mercuri Maurizio,
Pereira Amanda Francielli,
Pinheiro Ana Lucia Tabarini Alves,
Silva Michelle Sabrina,
Facchini Gustavo,
Eberlin Samara
Publication year - 2020
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.12979
Subject(s) - human skin , melanin , melasma , depigmentation , medicine , tyrosinase , melanocyte , dermatology , chemistry , melanoma , biology , biochemistry , cancer research , enzyme , genetics
Background Melanin plays an important role in protecting the skin against the harmful effects of solar radiation, but its abnormal accumulation may become an aesthetic problem, such as melasma and age spots. Aims The aim of this study was to evaluate the antiangiogenic and whitening effects of a depigmentation formulation ( BLTX ) using an in vitro model of human cell and skin culture. Methods Human fibroblasts, keratinocytes or melanocytes were treated with BLTX and subjected to oxidative stress by UV radiation or inflammatory stress with IL ‐1α for quantification of melanin, tyrosinase, endothelin‐1, PAR ‐2, VEGF and iNOS . Fragments of human skin, from elective plastic surgery, were treated with BLTX and subjected to histological evaluation with hematoxylin/eosin associated with Fontana‐Masson technique for melanin view. A parametric method, the one‐way analysis of variance ( ANOVA ) followed by the Bonferroni test, was used to compare data among all groups. Results The results demonstrated that BLTX promotes a reduction in VEGF and iNOS protein synthesis in cultured dermal fibroblasts, indicating an antiangiogenic property. In relation to whitening effect, BLTX was able to reduce the production of melanin in both systems, melanocytes and human skin cultures. The depigmenting action was also revealed by decreasing the levels of endothelin‐1, PAR ‐2 and activity of tyrosinase, when compared to cultures exposed to UV radiation. Conclusion The results allow us to infer that BLTX presents an antiangiogenic effect indicating a role in the vascular component of melasma. Furthermore, the whitening property observed reinforces its use in the prevention and treatment of melasma.