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Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57 BL /6 mice
Author(s) -
Kim Young Eun,
Choi Hyung Chul,
Nam Gaewon,
Choi Bu Young
Publication year - 2019
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.12674
Subject(s) - dermal papillae , hair follicle , wnt signaling pathway , smad , cell growth , in vivo , chemistry , microbiology and biotechnology , hedgehog , transforming growth factor , endocrinology , biology , signal transduction , medicine , biochemistry
Summary Background Costunolide ( COS ), a naturally occurring sesquiterpene lactone, is known to exert anti‐inflammatory, antioxidant, and anticancer effects. This study was undertaken to investigate the effects of costunolide on the promotion of hair growth. Methods Real‐time cell analyzer ( RTCA ), measurement of 5α‐reductase activity, mRNA expression, and Western blotting were adopted to address whether COS can stimulate the proliferation of human hair follicle dermal papilla cells ( hHFDPC s). The effect of COS on in vivo hair growth was examined by reconstitution assay and shaven dorsal skin in C57 BL /6 mice. Results Costunolide significantly promoted the proliferation of hHFDPC s, which is comparable to that of tofacitinib. COS also inhibited the 5α‐reductase activity in hHFDPC s. While COS increased the level of β‐catenin and Gli1 mRNA and proteins, it suppressed transforming growth factor ( TGF )‐β1–induced phosphorylation of Smad‐1/5 in hHFDPC s. COS increased the number of cultured hHFDPC s to induce hair follicles from mouse epidermal cells in Spheres formation of reconstitution assay. Topical application of COS on the shaven back of C57 BL /6 mice significantly improved the hair growth. Conclusions Our results illustrate that COS promotes hair growth in vitro and in vivo by regulating the amount of growth factors and/or the activity of cellular responses through coordination of the WNT ‐β‐catenin, hedgehog‐Gli, and TGF ‐β1–Smad pathways.