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Overexpression of Drosha, DiGeorge syndrome critical region gene 8 ( DGCR 8), and Dicer mRNA s in the pathogenesis of psoriasis
Author(s) -
Rostami Mogaddam Majid,
Safavi Ardabili Nastaran,
Shafaeei Yousef,
Maleki Nasrollah,
Jafari Naser,
Jafari Alireza
Publication year - 2017
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.12336
Subject(s) - drosha , dicer , psoriasis , downregulation and upregulation , pathogenesis , microrna , biology , cancer research , microbiology and biotechnology , immunology , pathology , medicine , gene , rna , genetics , rna interference
Summary Introduction Psoriasis is a complex autoimmune inflammatory disease that occurs in genetically susceptible individuals and presents with the development of inflammatory plaques on the skin. Recent studies have indicated that microRNAs (miRNAs) play important roles in psoriasis. Objective To investigate whether expression of Drosha, DGCR 8, and Dicer mRNA s is involved in the pathogenesis of psoriasis. Methods Biopsies were obtained from involved psoriatic skin ( PP ), noninvolved psoriatic skin ( PN ), and healthy skin ( NN ). Expression of Drosha, Dicer, and DGCR 8 was assessed with real‐time quantitative real‐time PCR in 25 patients with psoriasis and 25 healthy volunteers. Results We observed that expression levels of Drosha, Dicer, and DGCR 8 were upregulated in patients with psoriasis compared to the control group. However, the Drosha and Dicer expression levels were higher in PP tissues and PN tissues compared to NN tissues, but they were more upregulated in PP tissues compared to PN tissues ( P <.001). Although the DGCR 8 expression was higher in PP tissues and PN tissues compared to NN tissues, it was more upregulated in PN tissues compared to PP tissues ( P <.001). Conclusion Our data demonstrate that upregulated expression of Drosha, DGCR 8, and Dicer mRNA s may be involved in the pathogenesis of psoriasis.