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In vitro and clinical evaluation of SIG1273: a cosmetic functional ingredient with a broad spectrum of anti‐aging and antioxidant activities
Author(s) -
Fernández José R.,
Rouzard Karl,
Voronkov Michael,
Huber Kristen L.,
Webb Corey,
Stock Jeffry B.,
Stock Maxwell,
Gordon Joel S.,
Pérez Eduardo
Publication year - 2016
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.12206
Subject(s) - photoaging , antioxidant , chemistry , abts , pharmacology , oxidative stress , in vitro , wound healing , hydrogen peroxide , human skin , trolox , dermal fibroblast , skin aging , food science , biochemistry , medicine , dermatology , fibroblast , surgery , biology , dpph , genetics
Summary Background Isoprenylcysteine (IPC) small molecules were identified as a new class of anti‐inflammatory compounds over 20 years ago. Since then, they have been developed as novel cosmetic functional ingredients (CFI) and topical drug candidates. SIG1273 is a second generation CFI that has previously been shown to provide a broad spectrum of benefits for the skin through its anti‐inflammatory and antimicrobial properties. Objective To determine whether SIG1273 possesses anti‐aging properties in vitro and evaluate the tolerability and activity of SIG1273 when applied topically to human subjects. Methods To model photoaging in vitro , human dermal fibroblasts (HDFs) were exposed in culture to UVA to induce collagenase (MMP‐1) production. An in vitro wound‐healing model was based on the activation of HDF migration into cell‐free tissue culture surface. Hydrogen peroxide‐induced oxidative stress was performed using HDFs to measure intracellular ROS activity. Radical scavenging capacity was determined using a colorimetric antioxidant assay kit (ABTS method). Lastly, a 4‐week, 29‐subject study was performed in which SIG1273 was applied topically as a cream to assess its tolerance and activity in reducing the appearance of aging. Results In vitro studies demonstrate SIG1273 inhibits UVA‐induced MMP‐1 production, hydrogen peroxide‐induced oxidative stress and promotes wound healing. Moreover, SIG1273 was shown to be a radical scavenging antioxidant. Clinical assessment of SIG1273 cream (0.25%) showed it was well tolerated with significant improvement in the appearance of fine lines, coarse wrinkles, radiance/luminosity, pore size, texture/smoothness, hydration and increased firmness. Conclusions SIG1273 represents a novel CFI with antioxidant, anti‐aging, and anti‐inflammatory properties that when applied topically is well tolerated and provides benefits to individuals with aging skin.