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Protective effects of skin permeable epidermal and fibroblast growth factor against ultraviolet‐induced skin damage and human skin wrinkles
Author(s) -
An Jae Jin,
Eum Won Sik,
Kwon Hyuck Se,
Koh Jae Sook,
Lee Soo Yun,
Baek Ji Hwoon,
Cho YongJun,
Kim Dae Won,
Han Kyu Huyng,
Park Jinseu,
Jang Sang Ho,
Choi Soo Young
Publication year - 2013
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/jocd.12067
Subject(s) - fibroblast , wrinkle , human skin , epidermal growth factor , fibroblast growth factor , immunohistochemistry , skin repair , skin aging , wound healing , keratinocyte , dermal fibroblast , chemistry , microbiology and biotechnology , pathology , immunology , medicine , biology , dermatology , biochemistry , receptor , in vitro , genetics , gerontology
Summary Background Epidermal and fibroblast growth factor ( EGF and FGF 1) proteins play an important role in the regeneration and proliferation of skin cells. EGF and FGF 1 have considerable potential as possible therapeutic or cosmetic agents for the treatment of skin damage including wrinkles. Objectives Using protein transduction domains ( PTD ), we investigated whether PTD ‐ EGF and FGF 1 transduced into skin cells and tissue. Transduced proteins showed protective effects in a UV ‐induced skin damage model as well as against skin wrinkles. Methods Transduced PTD ‐ EGF and FGF 1 proteins were detected by immunofluorescence and immunohistochemistry. The effects of PTD ‐ EGF and FGF 1 were examined by WST assay, W estern blotting, immunohistochemistry, and skin wrinkle parameters. Results The PTD ‐ EGF and FGF 1 increased cell proliferation and collagen type 1 alpha 1 protein accumulation in skin tissue. Also, PTD ‐ EGF and FGF 1 inhibited UV ‐induced skin damage. Furthermore, topical application of PTD ‐ EGF and FGF 1 contained ampoules which were considered to improve the wrinkle parameters of human skin. Conclusion These results show that PTD ‐ EGF and FGF 1 can be a potential therapeutic or cosmetic agent for skin damaged and injury including wrinkles and aging.