Premium
The influence of castration on intramural neurons of the urinary bladder trigone in male pigs
Author(s) -
Kaleczyc Jerzy,
Sienkiewicz Waldemar,
Lepiarczyk Ewa,
KasicaJarosz Natalia,
Pidsudko Ze
Publication year - 2021
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.13450
Subject(s) - trigone of urinary bladder , castration , cholinergic , urinary bladder , adrenergic , endocrinology , medicine , immunohistochemistry , prostate , androgen , biology , receptor , cancer , hormone
The present study investigated the influence of castration performed at neonatal age on neuronal elements in the intramural ganglia of the urinary bladder trigone (UBT) in male pigs using double‐labeling immunohistochemistry. The ganglia were examined in intact (IP) 7‐day‐old (castration day) pigs, and at 3 and 6 months after surgery. In IP and control (3‐ and 6‐month‐old noncastrated pigs) groups, virtually, all neurons were adrenergic (68%) or cholinergic (32%) in nature. Many of them (32%, 51%, and 81%, respectively; 56%, 75%, and 85% adrenergic; and 32%, 52%, and 65% cholinergic, respectively) stained for the androgen receptor (AR), and only a small number of nerve cells were caspase‐3 (CASP‐3)‐positive. In 3‐ and 6‐month‐old castrated pigs, an excessive loss (87.6% and 87.5%, respectively) of neurons and intraganglionic nerve fibers was observed. The majority of the surviving adrenergic (61% and 72%, respectively) and many cholinergic (41% and 31%, respectively) neurons expressed CASP‐3 and were also AR‐positive (61% and 66%, and 40% and 36%, respectively). This study revealed for the first time the excessive loss of intramural UBT neurons following castration, which could have resulted from apoptosis induced by androgen deprivation.