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The distribution and density of monocarboxylate transporter 2 in cerebral cortex, hippocampus and cerebellum of wild‐type mice
Author(s) -
Pang Ruiqi,
Wang Xiaofan,
Du Zhiqiang,
Pei Feifei,
Li Zhiyue,
Sun Libing,
Wang Shuying,
Peng Yingnan,
Lu Xupeng,
Gao Xiaoqun,
Chang Cheng
Publication year - 2020
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.13099
Subject(s) - monocarboxylate transporter , hippocampus , immunogold labelling , purkinje cell , cerebellum , biology , cerebral cortex , western blot , medicine , endocrinology , transporter , neuroscience , microbiology and biotechnology , biochemistry , anatomy , gene , ultrastructure
Abstract Monocarboxylates cannot cross the blood‐brain barrier freely to participate in brain energy metabolism. Specific monocarboxylate transporters ( MCT s) are needed to cross cellular membranes. Monocarboxylate transporter 2 ( MCT 2) is a major monocarboxylate transporter encoded by the SLC 16A7 gene. Recent studies reported that neurodegenerative diseases of the CNS , such as Alzheimer's disease ( AD ) and Parkinson's disease ( PD ), were related to energy metabolic impairment. MCT 2 also plays an important role in energy metabolism in the CNS . To provide experimental evidence for future research on the role of MCT 2 in the pathological process of CNS degenerative diseases, the distribution and density of MCT 2 in different subregions of wild‐type mouse brain was examined using immunohistochemistry, western blot and immunogold post‐embedding electron microscopic techniques. The amount of MCT 2 was higher in cerebellum than in cortex and hippocampus on western blots, and there was no statistical difference between cortex and hippocampus. Immunohistochemistry assay revealed the highest density of MCT 2 in the CA 3 of the hippocampus. The granular cell layer of the cerebellum contained more MCT 2 than the molecular layer. The MCT 2 density on the end feet of astrocytes of molecular layer was lower than in hippocampus, but the postsynaptic densities ( PSD s) of asymmetric synapses in the molecular layer exhibited a high density using immunogold post‐embedding electron microscopic techniques.