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Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development
Author(s) -
Culjat Marko,
Milošević Nataša Jovanov
Publication year - 2019
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.13011
Subject(s) - corpus callosum , magnetic resonance imaging , anatomy , neuroscience , ex vivo , biology , immunohistochemistry , in vivo , pathology , medicine , radiology , microbiology and biotechnology
The early development and growth of the corpus callosum are supported by several midline transient structures in mammals that include callosal septa ( CS ), which are present only in the second half of gestation in humans. Here we provide new data that support the guidance role of CS in corpus callosum development, derived from the analysis of 46 postmortem fetal brains, ranging in age from 16 to 40 post conception weeks ( PCW ). Using immunohistochemical methods, we show the expression pattern of guidance cues ephrinA4 and neogenin, extracellular protein fibronectin, as well as non‐activated microglia in the CS . We found that the dynamic changes in expression of guidance cues, cellular and extracellular matrix constituents in the CS correlate well with the growth course of the corpus callosum at midsagittal level. The CS reach and maintain their developmental maximum between 20 and 26 PCW and can be visualized as hypointense structures in the ventral callosal portion with ex vivo ( in vitro ) T2‐weighted 3T magnetic resonance imaging ( MRI ). The maximum of septal development overlaps with an increase in the callosal midsagittal area, whereas the slow, gradual resolution of CS coincides with a plateau of midsagittal callosal growth. The recognition of CS existence in human fetal brain and the ability to visualize them by ex vivo MRI attributes a potential diagnostic value to these transient structures, as advancement in imaging technologies will likely also enable in vivo MRI visualization of the CS in the near future.