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Intraganglionic macrophages: a new population of cells in the enteric ganglia
Author(s) -
Dora David,
Arciero Emily,
Hotta Ryo,
Barad Csilla,
Bhave Sukhada,
Kovacs Tamas,
Balic Adam,
Goldstein Allan M.,
Nagy Nandor
Publication year - 2018
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.12863
Subject(s) - biology , green fluorescent protein , enteric nervous system , major histocompatibility complex , microglia , population , microbiology and biotechnology , antigen , haematopoiesis , immunology , stem cell , neuroscience , gene , medicine , genetics , inflammation , environmental health
The enteric nervous system shares embryological, morphological, neurochemical, and functional features with the central nervous system. In addition to neurons and glia, the CNS includes a third component, microglia, which are functionally and immunophenotypically similar to macrophages, but a similar cell type has not previously been identified in enteric ganglia. In this study we identify a population of macrophages in the enteric ganglia, intermingling with the neurons and glia. These intraganglionic macrophages ( IM s) are highly ramified and express the hematopoietic marker CD 45, major histocompatibility complex ( MHC) class II antigen, and chB6, a marker specific for B cells and microglia in avians. These IM s do not express antigens typically associated with T cells or dendritic cells. The CD 45 + /ChB6 + / MHCII + signature supports a hematopoietic origin and this was confirmed using intestinal chimeras in GFP ‐transgenic chick embryos. The presence of green fluorescent protein positive ( GFP +) / CD 45 + cells in the intestinal graft ENS confirms that IM s residing within enteric ganglia have a hematopoietic origin. IM s are also found in the ganglia of CSF 1 R GFP chicken and CX 3 CR 1 GFP mice. Based on the expression pattern and location of IM s in avians and rodents, we conclude that they represent a novel non‐neural crest‐derived microglia‐like cell population within the enteric ganglia.