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The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis
Author(s) -
Filipović Branko,
ŠošićJurjević Branka,
Ajdžanović Vladimir,
Pantelić Jasmina,
Nestorović Nataša,
Milošević Verica,
Sekulić Milka
Publication year - 2013
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.12013
Subject(s) - calcitonin , endocrinology , medicine , osteoporosis , osteocalcin , testosterone (patch) , androgen deficiency , androgen , cancellous bone , thyroid , testosterone propionate , hormone , chemistry , pathology , alkaline phosphatase , biochemistry , enzyme
Androgen deficiency is one of the major factors leading to the development of osteoporosis in men. Since calcitonin ( CT ) is a potent antiresorptive agent, in the present study we investigated the effects of androgen deficiency and subsequent testosterone and estradiol treatment on CT ‐producing thyroid C cells, skeletal and hormonal changes in middle‐aged orchidectomized ( O rx) rats. Fifteen‐month‐old male W istar rats were either O rx or sham‐operated ( SO ). One group of O rx rats received 5 mg kg −1 b.w. testosterone propionate ( TP ) subcutaneously, while another group was injected with 0.06 mg kg −1 b.w. estradiol dipropionate ( EDP ) once a day for 3 weeks. A peroxidase–antiperoxidase method was applied for localization of CT in the C cells. The studies included ultrastructural microscopic observation of these cells. The metaphyseal region of the proximal tibia was measured histomorphometrically using an imagej public domain image processing program. TP or EDP treatment significantly increased C cell volume ( V c), volume densities ( V v) and serum CT concentration compared with the O rx animals. Administration of both TP and EDP significantly enhanced cancellous bone area ( B . A r), trabecular thickness ( T b. T h) and trabecular number ( T b. N ) and reduced trabecular separation ( T b. S p). Serum osteocalcin ( OC ) and urinary C a concentrations were significantly lower after these treatments in comparison with O rx rats. These data suggest that testosterone and estradiol treatment in O rx middle‐aged rats affect calcitonin‐producing thyroid C cells, which may contribute to the bone protective effects of sex hormones in the rat model of male osteoporosis.