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Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats
Author(s) -
Puntel Gustavo O.,
Carvalho Nélson R.,
Dobrachinski Fernando,
Salgueiro Andréia C. F.,
Puntel Robson L.,
Folmer Vanderlei,
Barbosa Nilda B. V.,
Royes Luiz F. F.,
Rocha João Batista T.,
Soares Félix A. A.
Publication year - 2013
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/joa.12009
Subject(s) - cryotherapy , ischemia , skeletal muscle , medicine , thiobarbituric acid , catalase , chemistry , surgery , oxidative stress , lipid peroxidation
The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused ( I / R ) gastrocnemius muscle of rats. Forty male W istar rats were divided into control and I / R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid‐reactive substances and dichlorofluorescein oxidation levels were observed in I / R muscle. Cryotherapy in I / R muscle also minimized effects such as decreased cellular viability, levels of non‐protein thiols and calcium ATP ase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I / R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I / R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction.