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Nesfatin‐1 is an inhibitor of the growth hormone‐insulin‐like growth factor axis in goldfish ( Carassius auratus )
Author(s) -
Blanco Ayelén M.,
Pemberton Joshua G.,
Gonzalez Ronald,
Hatef Azadeh,
Pham Vi,
Chang John P.,
Unniappan Suraj
Publication year - 2021
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/jne.13010
Subject(s) - medicine , endocrinology , somatotropic cell , carassius auratus , autocrine signalling , pituitary gland , growth factor , biology , messenger rna , insulin like growth factor , adrenomedullin , hormone , chemistry , fish <actinopterygii> , receptor , gene , biochemistry , fishery
Nesfatin‐1, an 82 amino acid peptide cleaved from the N‐terminal of its precursor nucleobindin‐2 (NUCB2), is emerging as a multifunctional peptide in fish. The present study aimed to determine whether nesfatin‐1 plays a role in fish somatic growth by modulating the growth hormone (GH)/insulin‐like growth factor (IGF) axis, using a representative teleost model, the goldfish ( Carassius auratus ). The results demonstrated that a single i.p. injection of synthetic goldfish nesfatin‐1 significantly decreased the expression of hypothalamic pacap (approximately 90%) and pituitary Gh (approximately 90%) mRNAs at 15 minutes post‐injection. Serum GH levels were also reduced as a result of nesfatin‐1 administration, by approximately 45% and 55% at 15 and 30 minutes post‐injection, respectively. Likewise, in vitro treatment of goldfish dispersed pituitary cells with nesfatin‐1 reduced Gh secretion, suggesting that nesfatin‐1 acts directly on pituitary somatotrophs to inhibit Gh release. Exposure of cultured liver fragments to nesfatin‐1 (0.1, 1 and 10 nmol L ‐1 ) led to a significant reduction in igf‐1 mRNA at 120 minutes and of igf‐II mRNA at 30 and 60 minutes post‐incubation. Collectively, these results indicate a suppressive role for nesfatin‐1 on the goldfish GH/IGF axis. Immunohistochemical studies demonstrated that NUCB2/nesfatin‐1‐like immunoreactivity, although present in the goldfish pituitary, is not colocalised with GH in goldfish somatotrophs. Thus, nesfatin‐1 does not appear to act in an autocrine manner to regulate GH secretion. Taken together, this research found that the pituitary gland is an important source of endogenous NUCB2/nesfatin‐1 and also that nesfatin‐1 directly suppresses the Gh/IGF axis in goldfish.