z-logo
Premium
Neural androgen receptors affect the number of surviving new neurones in the adult dentate gyrus of male mice
Author(s) -
SwiftGallant A.,
DuarteGuterman P.,
Hamson D. K.,
Ibrahim M.,
Monks D. A.,
Galea L. A. M.
Publication year - 2018
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/jne.12578
Subject(s) - dentate gyrus , neurogenesis , medicine , endocrinology , androgen receptor , dihydrotestosterone , androgen , biology , hippocampal formation , hippocampus , bromodeoxyuridine , genetically modified mouse , neural stem cell , receptor , transgene , immunohistochemistry , neuroscience , microbiology and biotechnology , stem cell , hormone , prostate cancer , biochemistry , cancer , gene
Adult hippocampal neurogenesis occurs in many mammalian species. In rats, the survival of new neurones within the hippocampus is modulated by the action of androgen via the androgen receptor ( AR ); however, it is not known whether this holds true in mice. Furthermore, the evidence is mixed regarding whether androgens act in neural tissue or via peripheral non‐neural targets to promote new neurone survival in the hippocampus. We evaluated whether the action of androgen via AR underlies the survival of new neurones in mice, and investigated whether increasing AR selectively in neural tissue would increase new neurone survival in the hippocampus. We used the cre‐loxP system to overexpress AR only in neural tissues (Nestin‐ AR ). These males were compared with wild‐type males, as well as control males with 1 of the 2 mutations required for overexpression. Mice were gonadectomised and injected with the DNA synthesis marker, bromodeoxyuridine (BrdU) and for 37 days (following BrdU injection), mice were treated with oil or dihydrotestosterone ( DHT ). Using immunohistochemistry, proliferation (Ki67) and survival (BrdU) of new neurones were both evaluated in the dorsal and ventral dentate gyrus. Dihydrotestosterone treatment increased the survival of new neurones in the entire hippocampus in wild‐type mice and control mice that only have 1 of 2 necessary mutations for transgenic expression. However, DHT treatment did not increase the survival of new neurones in mice that overexpressed AR in neural tissue. Cell proliferation (Ki67) and cell death (pyknotic cells) were not affected by DHT treatment in wild‐type or transgenic males. These results suggest that androgens act via neural AR to affect hippocampal neurogenesis by promoting cell survival; however, the relationship between androgen dose and new neurone survival is nonlinear.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here