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Sex Hormones Protect Against Amyloid‐β Induced Oxidative Stress in the Choroid Plexus Cell Line Z310
Author(s) -
Costa A. R.,
Marcelino H.,
Gonçalves I.,
Quintela T.,
Tomás J.,
Duarte A. C.,
Fonseca A. M.,
Santos C. R. A.
Publication year - 2016
Publication title -
journal of neuroendocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.062
H-Index - 116
eISSN - 1365-2826
pISSN - 0953-8194
DOI - 10.1111/jne.12404
Subject(s) - oxidative stress , choroid plexus , neuroprotection , endocrinology , medicine , hormone , leydig cell , biology , cerebrospinal fluid , microbiology and biotechnology , chemistry , central nervous system , luteinizing hormone
The choroid plexus ( CP ) epithelium is a unique structure in the brain that forms an interface between the peripheral blood on the basal side and the cerebrospinal fluid ( CSF ) on the apical side. It is a relevant source of many polypeptides secreted to the CSF with neuroprotective functions and also participates in the elimination and detoxification of brain metabolites, such as β‐amyloid (Aβ) removal from the CSF through transporter‐mediated influx. The CP is also a target tissue for sex hormones ( SH s) that have recognised neuroprotective effects against a variety of insults, including Aβ toxicity and oxidative stress in the central nervous system. The present study aimed to understand how SH s modulate Aβ‐induced oxidative stress in a CP cell line (Z310 cell line) by analysing the effects of Aβ 1–42 on oxidative stress, mitochondrial function and apoptosis, as well as by assessing how 17β‐oestradiol (E 2 ) and 5α‐dihydrotestosterone ( DHT ) modulated these effects and the cellular uptake of Aβ 1–42 by CP cells. Our findings show that E 2 and DHT treatment reduce Aβ 1–42 ‐induced oxidative stress and the internalisation of Aβ 1–42 by CP epithelial cells, highlighting the importance of considering the background of SH s and therefore sex‐related differences in Aβ metabolism and clearance by CP cells.