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Neuronal mechanisms of adenosine A 2A receptors in the loss of consciousness induced by propofol general anesthesia with functional magnetic resonance imaging
Author(s) -
Chen Lei,
Li Shuang,
Zhou Ying,
Liu Taotao,
Cai Aoling,
Zhang Zongze,
Xu Fuqiang,
Manyande Anne,
Wang Jie,
Peng Mian
Publication year - 2021
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.15146
Subject(s) - propofol , nucleus accumbens , adenosine a2a receptor , functional magnetic resonance imaging , anesthesia , neuroscience , anesthetic , medicine , adenosine , agonist , pharmacology , adenosine receptor , chemistry , psychology , receptor
Propofol is the most common intravenous anesthetic agent for induction and maintenance of anesthesia, and has been used clinically for more than 30 years. However, the mechanism by which propofol induces loss of consciousness (LOC) remains largely unknown. The adenosine A 2A receptor (A 2A R) has been extensively proven to have an effect on physiological sleep. It is, therefore, important to investigate the role of A 2A R in the induction of LOC using propofol. In the present study, the administration of the highly selective A 2A R agonist (CGS21680) and antagonist (SCH58261) was utilized to investigate the function of A 2A R under general anesthesia induced by propofol by means of animal behavior studies, resting‐state magnetic resonance imaging and c ‐Fos immunofluorescence staining approaches. Our results show that CGS21680 significantly prolonged the duration of LOC induced by propofol, increased the c ‐Fos expression in nucleus accumbens (NAc) and suppressed the functional connectivity of NAc‐dorsal raphe nucleus (DR) and NAc‐cingulate cortex (CG). However, SCH58261 significantly shortened the duration of LOC induced by propofol, decreased the c ‐Fos expression in NAc, increased the c ‐Fos expression in DR, and elevated the functional connectivity of NAc‐DR and NAc‐CG. Collectively, our findings demonstrate the important roles played by A 2A R in the LOC induced by propofol and suggest that the neural circuit between NAc‐DR maybe controlled by A 2A R in the mechanism of anesthesia induced by propofol.