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Issue Cover (January 2020)
Publication year - 2020
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.14727
Subject(s) - antibody , flow cytometry , citation , cover (algebra) , computer science , phosphorylation , artificial intelligence , information retrieval , computational biology , microbiology and biotechnology , chemistry , medicine , library science , biology , immunology , biochemistry , mechanical engineering , engineering
Front cover: Phospho‐tau antibodies are the most common tool for detecting site‐specific tau phosphorylation. However, there is no quantitative measure of specificity that guides users. We developed a robust flow cytometry assay that determines a specificity parameter termed ϕ(phi), which measures the fraction of non‐specific signal in antibody binding. We applied the assay to measure the specificity of widely used phospho‐tau antibodies, and show that phospho‐tau antibodies AT8, AT180, PHF‐6, TG‐3, and PHF‐1 have nearly perfect specificity. Image content: Representative confocal image showing highly specific recognition of tau phosphorylated at Thr231 (red) among total tau (green), imaged using EGFP‐tau.Read the full preface ‘High specificity of widely used phospho‐tau antibodies validated using a quantitative wholecell based assay’ by D. Li, Y. K. Cho ( J. Neurochem . 2020, vol. 152 (1), pp. 122–135) on doi: 10.1111/jnc.14830