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Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas
Author(s) -
Shimizu Takeshi,
Osanai Yasuyuki,
Tanaka Kenji F.,
Thai Truc Quynh,
Abe Manabu,
Natsume Rie,
Sakimura Kenji,
Ikenaka Kazuhiro
Publication year - 2019
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.14657
Subject(s) - vinculin , microbiology and biotechnology , gene knockdown , focal adhesion , chemistry , morphogenesis , biology , phosphorylation , biochemistry , gene
Abstract Oligodendrocytes ( OL s) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However, the molecular mechanisms underlying the mechanical regulation of OL s by mechanosensors remain largely unknown. We found that morphology of OL was affected by knockdown of the mechanosensors p130Cas or talin1. Stretching of OL precursor cells induced the phosphorylation of p130Cas and talin‐associated assembly of vinculin. Shear stress decreased the number of OL processes, whereas these effects were mechanically suppressed by dominant‐negative ( DN ) p130Cas, but not by DN ‐talin1. To investigate the roles of p130Cas in post‐natal OL s in vivo , we constructed a novel p130Cas knock‐in mouse and found overexpression of p130Cas in vivo affected the number of mature OL s in the cortex. These results indicate that the mechanosensor p130Cas controls both OL morphogenesis and maturation.