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Clenbuterol reduces GABA ergic transmission in prefrontal cortex layer 5/6 pyramidal neurons of juvenile rat via reducing action potentials firing frequency of GABA ergic interneurons
Author(s) -
Luo F.,
Zhou H.
Publication year - 2018
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.14248
Subject(s) - hyperpolarization (physics) , neuroscience , chemistry , neurotransmission , gabaergic , hcn channel , biophysics , inhibitory postsynaptic potential , ion channel , biology , receptor , biochemistry , organic chemistry , nuclear magnetic resonance spectroscopy
Beta‐adrenoceptors (β 2 ‐ AR s) have beneficial effects on prefrontal cortex ( PFC ) working memory, however, the cellular and molecular mechanisms are unclear yet. In this study, we probed the effect of β 2 ‐ AR ‐selective agonist clenbuterol (Clen) on synaptic transmission in layer 5/6 pyramidal neurons of PFC . Bath application of Clen reduced spontaneous IPSC ( sIPSC ) frequency without effects on sEPSC s. Clen did not alter the frequency and amplitude of miniature IPSC s ( mIPSC s), but exerted heterogeneous effects on evoked IPSC s ( eIPSC s) recorded from PFC layer 5/6 pyramidal neurons. Clen decreased the firing rate of action potentials of fast‐spiking GABA ergic interneurons. Clen‐induced hyperpolarization of fast‐spiking GABA ergic interneurons required potentiation of an inward rectifier K + channels. Clen‐induced hyperpolarization of fast‐spiking interneurons was dependent on Gs protein rather than cAMP and protein kinase A. Our findings demonstrate that Clen (10 μM) enhances inward rectifier K + channels via Gs protein to cause membrane hyperpolarization of fast‐spiking GABA ergic interneurons resulting in reduction of action potentials firing rate to reduce GABA ergic transmission.