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Retracted: Intra‐arterial transplantation of human bone marrow mesenchymal stem cells ( hBMMSC s) improves behavioral deficits and alters gene expression in rodent stroke model
Author(s) -
Khan Rehan,
Sharma Alpana,
Jain Suman,
Mohanty Sujata,
Prasad Kameshwar
Publication year - 2017
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.14241
Subject(s) - mesenchymal stem cell , stem cell , bone marrow , transplantation , stroke (engine) , neurotrophic factors , bone marrow stem cell , biology , gene expression , medicine , gene , pathology , endocrinology , microbiology and biotechnology , genetics , mechanical engineering , receptor , engineering
Stroke is a multi‐factorial polygenic disease and is a major cause of death and adult disability. Administration of bone marrow stem cells protects ischemic rat brain by facilitating recovery of neurological functions. But the molecular mechanism of stem cells action and their effect on gene expression is not well explored. In this study, we have transplanted 1 × 10 6 human bone marrow mesenchymal stem cells ( hBMMSC s) in middle cerebral artery occluded ( MCA o) adult male Wistar rats through intracarotid artery route at 24 h after surgery. Motor behavioral tests (rotarod and open field) were performed to assess the changes in motor functions at day 0 and day1, 4, 8 and 14. The expression of studied genes at mRNA and protein level was quantified by using Q‐ PCR and western blotting, respectively. Further, we have assessed the methylation pattern of promoter of these genes by using methylation‐specific PCR . Data were analyzed statistically and correlated. A significant improvement in behavioral deficits was observed in stem cells treated group after 14th day post stroke. Significantly ( p < 0.05) increased mRNA and protein levels of brain derived neurotrophic factor and ANP genes in hBMMSC s treated group along with decrease in methylation level at their promoter was observed. On the other hand, significantly decreased mRNA and protein level of TSP 1 and WNK 1 in hBMMSC s treated group was observed. In conclusion, hBMMSC s administration significantly improves the behavioral deficits by improving motor and locomotor coordination. The promoter of TSP 1 and WNK 1 genes was found to be hyper‐methylated in hBMMSC s group resulting in their decreased expression while the promoter of ANP and brain derived neurotrophic factor was found to be hypo‐methylated. This study might shed a light on how hBMMSC s affect the gene expression by modulating methylation status.