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Issue Cover (May 2018)
Publication year - 2018
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.14175
Subject(s) - glutamatergic , fibroblast growth factor , transgene , neuroscience , biology , gene , mcherry , psychology , genetics , green fluorescent protein , receptor , glutamate receptor
Front cover:Background: Fibroblast Growth Factor 2 (FGF2) gene expression has been demonstrated to be altered in brain regions of major depressive disorder (MDD) patients, and FGF2 has been shown to have anti‐depressive effects in animal models of depression. Main finding: Glutamatergic neurons (iNeurons) were derived from human stem cells using the stable integration of a human NEUROG2 transgene. This system was used to demonstrate that FGF2 enhances glutamatergic transmission in these iNeurons. Several FGF2‐regulated genes were identified mediating this enhancement including NRNX3 and SYT2. Implication: Since several of these genes have been implicated in MDD previously, these results will provide the basis for more mechanistic studies of the role of FGF2 in MDD. Image Content: This image shows iNeurons derived from human stem cells that have been labeled with ubiquitous mCherry (red) expression and GCamp6f (green) as a reporter of calcium activity. A high ratio of green to red fluorescence is indicative of high neuronal calcium levels.Read the full article ‘Fibroblast growth factor 2 regulates activity and gene expression of human post‐mitotic excitatory neurons’ by S. Gupta, T. M‐Redmond, F. Meng, A. Tidball, H. Akil, S. Watson, J. M. Parent, M. Uhler ( J. Neurochem. 2018, vol. 145 (3), pp. 188–203) on doi: 10.1111/jnc.14255