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The oligodendrocyte‐specific antibody ‘ CC 1’ binds Quaking 7
Author(s) -
Bin Jenea M.,
Harris Stephanie N.,
Kennedy Timothy E.
Publication year - 2016
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.13745
Subject(s) - oligodendrocyte , antibody , clone (java method) , monoclonal antibody , microbiology and biotechnology , biology , antigen , adenomatous polyposis coli , myelin , rna binding protein , escherichia coli , rna , biochemistry , central nervous system , immunology , genetics , dna , gene , cancer , colorectal cancer , neuroscience
The mouse monoclonal antibody marketed as anti‐adenomatous polyposis coli clone CC 1, often referred to as CC 1, is the antibody most commonly used to specifically label mature oligodendrocytes without labeling myelin. Previous studies have shown that despite being raised against adenomatous polyposis coli, this antibody binds another unknown antigen. We show that the CC 1 antibody binds Quaking 7, an RNA ‐binding protein that is highly up‐regulated in myelinating oligodendrocytes in the central nervous system.The monoclonal antibody anti‐adenomatous polyposis coli (APC) clone CC1, is the antibody most commonly used to specifically label the cell bodies of mature oligodendrocytes. Despite being raised against APC, previous studies showed this antibody binds another unknown antigen. We show that the CC1 antibody binds Quaking (QKI) 7, an RNA‐binding protein which is highly up‐regulated in myelinating oligodendrocytes.