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Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule‐related proteins in neurons
Author(s) -
Kenney Justin W.,
Genheden Maja,
Moon KyungMee,
Wang Xuemin,
Foster Leonard J.,
Proud Christopher G.
Publication year - 2016
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.13407
Subject(s) - elongation factor , microtubule , microbiology and biotechnology , biology , protein biosynthesis , tubulin , elongation , stable isotope labeling by amino acids in cell culture , cytoskeleton , eukaryotic translation elongation factor 1 alpha 1 , biochemistry , cell , proteomics , gene , rna , ribosome , materials science , ultimate tensile strength , metallurgy
Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in both neurons and other cell types. Elongation is primarily regulated via eukaryotic elongation factor 2 kinase ( eEF 2K). However, the consequence of altering eEF 2K activity on the synthesis of specific proteins is largely unknown. Using both pharmacological and genetic manipulations of eEF 2K combined with two protein‐labeling techniques, stable isotope labeling of amino acids in cell culture and bio‐orthogonal non‐canonical amino acid tagging, we identified a subset of proteins whose synthesis is sensitive to inhibition of eEF 2K in murine primary cortical neurons. Gene ontology ( GO ) analyses indicated that processes related to microtubules are particularly sensitive to eEF 2K inhibition. Our findings suggest that eEF 2K likely contributes to neuronal function by regulating the synthesis of microtubule‐related proteins.Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in neurons. Here, using labeling of new proteins coupled with proteomic techniques in primary cortical neurons, we find that the synthesis of microtubule‐related proteins is up‐regulated by inhibition of elongation. This suggests that translation elongation is a key regulator of cytoskeletal dynamics in neurons.