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Differential peptidomics assessment of strain and age differences in mice in response to acute cocaine administration
Author(s) -
Romanova Elena V.,
Rubakhin Stanislav S.,
Ossyra John R.,
Zombeck Jonathan A.,
Nosek Michael R.,
Sweedler Jonathan V.,
Rhodes Justin S.
Publication year - 2015
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.13265
Subject(s) - peptide , endocrinology , neurochemical , medicine , hypothalamus , neuropeptide , biology , chemistry , biochemistry , receptor
Neurochemical differences in the hypothalamic‐pituitary axis between individuals and between ages may contribute to differential susceptibility to cocaine abuse. This study measured peptide levels in the pituitary gland (Pit) and lateral hypothalamus ( LH ) in adolescent (age 30 days) and adult (age 65 days) mice from four standard inbred strains, FVB / NJ , DBA /2J, C57 BL /6J, and BALB / cByJ , which have previously been characterized for acute locomotor responses to cocaine. Individual peptide profiles were analyzed using mass spectrometric profiling and principal component analysis. Sequences of assigned peptides were verified by tandem mass spectrometry. Principal component analysis classified all strains according to their distinct peptide profiles in Pit samples from adolescent mice, but not adults. Select pro‐opiomelanocortin‐derived peptides were significantly higher in adolescent BALB / cByJ and DBA /2J mice than in FVB / NJ or C57 BL /6J mice. A subset of peptides in the LH , but not in the Pit, was altered by cocaine in adolescents. A 15 mg/kg dose of cocaine induced greater peptide alterations than a 30 mg/kg dose, particularly in FVB / NJ animals, with larger differences in adolescents than adults. Neuropeptides in the LH affected by acute cocaine administration included pro‐opiomelanocortin‐, myelin basic protein‐, and glutamate transporter‐derived peptides. The observed peptide differences could contribute to differential behavioral sensitivity to cocaine among strains and ages.Peptides were measured using mass spectrometry (MALDI‐TOF) in individual lateral hypothalamus and pituitary samples from four strains and two ages of inbred mice in response to acute cocaine administration. Principal component analyses (PCA) classified the strains according to their peptide profiles from adolescent mice, and a subset of peptides in the lateral hypothalamus was altered by cocaine in adolescents.