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Transplanted microvascular endothelial cells promote oligodendrocyte precursor cell survival in ischemic demyelinating lesions
Author(s) -
Iijima Keiya,
Kurachi Masashi,
Shibasaki Koji,
Naruse Masae,
Puentes Sandra,
Imai Hideaki,
Yoshimoto Yuhei,
Mikuni Masahiko,
Ishizaki Yasuki
Publication year - 2015
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.13262
Subject(s) - remyelination , transplantation , oligodendrocyte , apoptosis , white matter , pathology , in vivo , programmed cell death , biology , multiple sclerosis , medicine , immunology , magnetic resonance imaging , neuroscience , myelin , central nervous system , biochemistry , microbiology and biotechnology , radiology
We previously showed that transplantation of brain microvascular endothelial cells ( MVEC s) greatly stimulated remyelination in the white matter infarct of the internal capsule (IC) induced by endothelin‐1 injection and improved the behavioral outcome. In the present study, we examined the effect of MVEC transplantation on the infarct volume using intermittent magnetic resonance image and on the behavior of oligodendrocyte lineage cells histochemically. Our results in vivo show that MVEC transplantation reduced the infarct volume in IC and apoptotic death of oligodendrocyte precursor cells ( OPC s). These results indicate that MVEC s have a survival effect on OPC s, and this effect might contribute to the recovery of the white matter infarct. The conditioned‐medium from cultured MVEC s reduced apoptosis of cultured OPC s, while the conditioned medium from cultured fibroblasts did not show such effect. These results suggest a possibility that transplanted MVEC s increased the number of OPC s through the release of humoral factors that prevent their apoptotic death. Identification of such humoral factors may lead to the new therapeutic strategy against ischemic demyelinating diseases.Transplanted microvascular endothelial cells (MVECs) release humoral factors which increase the number of oligodendrocyte precursor cells (OPCs) by inhibiting their apoptotic death. The resultant increase in OPCs promotes remyelination by existing (intrinsic) mature oligodendrocytes in the ischemic infarct region. Identification of such humoral factors may lead to the new therapeutic strategy against ischemic demyelinating diseases.