Premium
Drebrin depletion alters neurotransmitter receptor levels in protein complexes, dendritic spine morphogenesis and memory‐related synaptic plasticity in the mouse hippocampus
Author(s) -
Jung Gangsoo,
Kim EunJung,
Cicvaric Ana,
Sase Sunetra,
Gröger Marion,
Höger Harald,
Sialana Fernando Jayson,
Berger Johannes,
Monje Francisco J.,
Lubec Gert
Publication year - 2015
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.13119
Subject(s) - dendritic spine , neurotransmitter , synaptic plasticity , dendrite (mathematics) , neuroscience , biology , hippocampal formation , receptor , microbiology and biotechnology , hippocampus , long term potentiation , glutamatergic , dopamine , glutamate receptor , biochemistry , central nervous system , geometry , mathematics
Drebrin an actin‐bundling key regulator of dendritic spine genesis and morphology, has been recently proposed as a regulator of hippocampal glutamatergic activity which is critical for memory formation and maintenance. Here, we examined the effects of genetic deletion of drebrin on dendritic spine and on the level of complexes containing major brain receptors. To this end, homozygous and heterozygous drebrin knockout mice generated in our laboratory and related wild‐type control animals were studied. Level of protein complexes containing dopamine receptor D1/dopamine receptor D2, 5‐hydroxytryptamine receptor 1A (5‐ HT 1 A R ), and 5‐hydroxytryptamine receptor 7 (5‐ HT 7R) were significantly reduced in hippocampus of drebrin knockout mice whereas no significant changes were detected for GluR1, 2, and 3 and NR 1 as examined by native gel‐based immunoblotting. Drebrin depletion also altered dendritic spine formation, morphology, and reduced levels of dopamine receptor D1 in dendritic spines as evaluated using immunohistochemistry/confocal microscopy. Electrophysiological studies further showed significant reduction in memory‐related hippocampal synaptic plasticity upon drebrin depletion. These findings provide unprecedented experimental support for a role of drebrin in the regulation of memory‐related synaptic plasticity and neurotransmitter receptor signaling, offer relevant information regarding the interpretation of previous studies and help in the design of future studies on dendritic spines.We examined effect of genetic deletion of drebrin, which an actin‐bundling key regulator of dendritic spine genesis and morphology, on dendritic spine density, maturity, level of complexes containing major brain receptors and also, in synaptic plasticity. These findings support for a role of drebrin in the regulation of memory‐related synaptic plasticity and neurotransmitter receptors signaling in dendritic spines.