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Hydrogen sulfide is essential for Schwann cell responses to peripheral nerve injury
Author(s) -
Park Byung Sun,
Kim HyunWook,
Rhyu Im Joo,
Park Chan,
Yeo Seung Geun,
Huh Youngbuhm,
Jeong Na Young,
Jung Junyang
Publication year - 2015
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12932
Subject(s) - wallerian degeneration , cystathionine beta synthase , schwann cell , sulfurtransferase , in vivo , microbiology and biotechnology , peripheral nerve injury , cystathionine gamma lyase , biology , chemistry , biochemistry , neuroscience , cysteine , enzyme , regeneration (biology)
Hydrogen sulfide (H 2 S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H 2 S is produced from substances by three enzymes: cystathionine β‐synthase (CBS), cystathionine γ‐lyase (CSE), and 3‐mercaptopyruvate sulfurtransferase (MST). In human tissues, these enzymes are involved in tissue‐specific biochemical pathways for H 2 S production. For example, CBS and cysteine aminotransferase/MST are present in the brain, but CSE is not. Thus, we examined the expression of H 2 S production‐related enzymes in peripheral nerves. Here, we found that CSE and MST/cysteine aminotransferase, but not CBS, were present in normal peripheral nerves. In addition, injured sciatic nerves in vivo up‐regulated CSE in Schwann cells during Wallerian degeneration (WD); however, CSE was not up‐regulated in peripheral axons. Using an ex vivo sciatic nerve explant culture, we found that the inhibition of H 2 S production broadly prevented the process of nerve degeneration, including myelin fragmentation, axonal degradation, Schwann cell dedifferentiation, and Schwann cell proliferation in vitro and in vivo . Thus, these results indicate that H 2 S signaling is essential for Schwann cell responses to peripheral nerve injury.Hydrogen sulfide (H 2 S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H 2 S is produced from cystathionine β‐synthase (CBS), cystathionine γ‐lyase (CSE), and 3‐mercaptopyruvate sulfur transferase (MST). Here, we found that CSE and MST/CAT were present in normal peripheral nerves. Injured static nerves in vivo up‐regulated CSE in Schwann cells during Wallerian degeneration, but CSE was not up‐regulated in peripheral axons.