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Ischemic neurons activate astrocytes to disrupt endothelial barrier via increasing VEGF expression
Author(s) -
Li YingNa,
Pan Rong,
Qin XuJun,
Yang WeiLin,
Qi Zhifeng,
Liu Wenlan,
Liu Ke Jian
Publication year - 2014
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12611
Subject(s) - blood–brain barrier , occludin , microbiology and biotechnology , endothelial stem cell , ischemia , tight junction , astrocyte , vascular permeability , vascular endothelial growth factor , biology , endothelium , chemistry , neuroscience , in vitro , endocrinology , medicine , cancer research , central nervous system , biochemistry , vegf receptors
Blood–brain barrier (BBB) disruption occurring within the first few hours of ischemic stroke onset is closely associated with hemorrhagic transformation following thrombolytic therapy. However, the mechanism of this acute BBB disruption remains unclear. In the neurovascular unit, neurons do not have direct contact with the endothelial barrier; however, they are highly sensitive and vulnerable to ischemic injury, and may act as the initiator for disrupting BBB when cerebral ischemia occurs. Herein, we employed oxygen–glucose deprivation (OGD) and an in vitro BBB system consisting of brain microvascular cells and astrocytes to test this hypothesis. Neurons (CATH.a cells) were exposed to OGD for 3‐h before co‐culturing with endothelial monolayer (bEnd 3 cells), or endothelial cells plus astrocytes (C8‐D1A cells). Incubation of OGD‐treated neurons with endothelial monolayer alone did not increase endothelial permeability. However, when astrocytes were present, the endothelial permeability was significantly increased, which was accompanied by loss of occludin and claudin‐5 proteins as well as increased vascular endothelial growth factor (VEGF) secretion into the conditioned medium. Importantly, all these changes were abolished when VEGF was knocked down in astrocytes by siRNA. Our findings suggest that ischemic neurons activate astrocytes to increase VEGF production, which in turn induces endothelial barrier disruption.Little is known about the contribution of neurons to blood–brain barrier (BBB) injury following cerebral ischemia. Using co‐culture model of neurons, astrocytes and endothelial cells, we found that ischemic neurons activated astrocytes to increase vascular endothelial growth factor (VEGF) secretion that in turns acted on tight junction proteins to increase BBB permeability. These results clearly suggest an important role of neurons in ischemic BBB damage.