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EFA 6A, a guanine nucleotide exchange factor for Arf6, interacts with sorting nexin‐1 and regulates neurite outgrowth
Author(s) -
Fukaya Masahiro,
Fukushima Daisuke,
Hara Yoshinobu,
Sakagami Hiroyuki
Publication year - 2014
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12524
Subject(s) - retromer , neurite , colocalization , sorting nexin , guanine nucleotide exchange factor , adp ribosylation factor , microbiology and biotechnology , endosome , vacuolar protein sorting , biology , clathrin , chemistry , golgi apparatus , endocytosis , signal transduction , biochemistry , receptor , endoplasmic reticulum , in vitro , intracellular
The membrane trafficking and actin cytoskeleton remodeling mediated by ADP ribosylation factor 6 (Arf6) are functionally linked to various neuronal processes including neurite formation and maintenance, neurotransmitter release, and receptor internalization. EFA 6A is an Arf6‐specific guanine nucleotide exchange factor that is abundantly expressed in the brain. In this study, we identified sorting nexin‐1 ( SNX 1), a retromer component that is implicated in endosomal sorting and trafficking, as a novel interacting partner for EFA 6A by yeast two‐hybrid screening. The interaction was mediated by the C‐terminal region of EFA 6A and a BAR domain of SNX 1, and further confirmed by pull‐down assay and immunoprecipitation from mouse brain lysates. In situ hybridization analysis demonstrated the widespread expression of SNX 1 in the mouse brain, which overlapped with the expression of EFA 6A in the forebrain. Immunofluorescent analysis revealed the partial colocalization of EFA 6A and SNX 1 in the dendritic fields of the hippocampus. Immunoelectron microscopic analysis revealed the overlapping subcellular localization of EFA 6A and SNX 1 at the post‐synaptic density and endosomes in dendritic spines. In Neuro‐2a neuroblastoma cells, expression of either EFA 6A or SNX 1 induced neurite outgrowth, which was further enhanced by co‐expression of EFA 6A and SNX 1. The present findings suggest a novel mechanism by which EFA 6A regulates Arf6‐mediated neurite formation through the interaction with SNX 1.We identified sorting nexin‐1 (SNX1) as a novel binding partner for EFA6A, and demonstrated overlapping ultrastructural localization of EFA6A and SNX1 in spines and dendrites of hippocampal neurons. Furthermore, we showed that this interaction enhanced neurite outgrowth of Neuro‐2a cells. The present findings suggest the importance of the interaction between EFA6A and SNX1 in Arf6‐mediated neuronal functions in dendritic spines.

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