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Genomic editing opens new avenues for zebrafish as a model for neurodegeneration
Author(s) -
Schmid Bettina,
Haass Christian
Publication year - 2013
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12460
Subject(s) - zebrafish , genome editing , transcription activator like effector nuclease , model organism , zinc finger nuclease , palindrome , biology , computational biology , effector , crispr , genome , genetics , organism , neurodegeneration , gene , microbiology and biotechnology , medicine , disease , pathology
Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator‐like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat‐associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock‐out and, importantly, also makes knock‐in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off‐target effects and toxicity as frequently seen in conventional knock‐down approaches.