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Stress‐induced brain histone H3 phosphorylation: contribution of the intensity of stressors and length of exposure
Author(s) -
Rotllant David,
PastorCiurana Jordi,
Armario Antonio
Publication year - 2013
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/jnc.12214
Subject(s) - epigenetics , histone , histone h3 , stressor , phosphorylation , c fos , serine , neuroscience , biology , microbiology and biotechnology , chemistry , medicine , gene expression , endocrinology , gene , biochemistry
Expression of c‐fos is used for the characterization of brain areas activated by stressors. Recently, some epigenetic markers associated with enhanced transcription have been identified that may be also useful to detect neuronal populations important for the processing of stressors: phosphorylation of histone H3 in serine 10 or 28 ( pH 3S 10 or pH 3S 28 ). Then, we compared in rats the response to stress of c‐fos and these epigenetic changes. More specifically, we studied the influence of the type of stressor (novel environment vs. immobilization, IMO) and the dynamics of the response to IMO. Stress increased pH 3S 10 positive neurons, with a more restricted pattern than that of c‐fos, both in terms of brain areas activated and number of positive neurons. Changes in pH 3S 10 showed a maximum at 30 min, then progressively declining in most areas in spite of the persistence of IMO. Moreover, the decline was in general more sensitive than c‐fos to the termination of IMO. The pattern of pH 3S 28 was even more restricted that of pH 3S 10 , but they showed co‐localization. The present data demonstrate a more selective pattern of stress‐induced histone H3 phosphorylation than c‐fos. The factors determining such a selectivity and its biological meaning remain to be studied.