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Efficiency of whole‐exome sequencing in old world and new world primates using human capture reagents
Author(s) -
Chan Jeannie,
Yao Wen,
Howard Timothy D.,
Hawkins Gregory A.,
Olivier Michael,
Jorgensen Matthew J.,
Cheeseman Ian H.,
Cole Shelley A.,
Cox Laura A.
Publication year - 2021
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12524
Subject(s) - exome sequencing , exome , biology , human genome , computational biology , gene , dna sequencing , genetics , genome , 1000 genomes project , genomics , single nucleotide polymorphism , mutation , genotype
Background Whole‐exome sequencing (WES) can expedite research on genetic variation in non‐human primate (NHP) models of human diseases. However, NHP‐specific reagents for exome capture are not available. This study reports the use of human‐specific capture reagents in WES for olive baboons, marmosets, and vervet monkeys. Methods Exome capture was carried out using the SureSelect Human All Exon V6 panel from Agilent Technologies, followed by high‐throughput sequencing. Capture of protein‐coding genes and detection of single nucleotide variants were evaluated. Results Exome capture and sequencing results showed that more than 97% of old world and 93% of new world monkey protein coding genes were detected. Single nucleotide variants were detected across the genomes and missense variants were found in genes associated with human diseases. Conclusions A cost‐effective approach based on commercial, human‐specific reagents can be used to perform WES for the discovery of genetic variants in these NHP species.