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Comparison of semen characteristics and sperm cryopreservation in common marmoset ( Callithrix jacchus ) and black‐tufted‐ear marmoset ( Callithrix penicillata )
Author(s) -
Arakaki Paloma Rocha,
Nichi Marcílio,
Monteiro Frederico Ozanan Barros,
Muniz José Augusto Pereira Carneiro,
Guimarães Marcelo Alcindo de Barros Vaz,
Valle Rodrigo del Rio do
Publication year - 2019
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12388
Subject(s) - marmoset , callithrix , biology , sperm , semen , semen collection , anatomy , andrology , zoology , artificial insemination , ecology , medicine , botany , pregnancy , genetics
Background The common marmoset ( Callithrix jacchus ) is one of the most studied Neotropical primates regarding reproduction. However, little has been reported on the black‐tufted‐ear marmoset ( Callithrix penicillata ), which may produce fertile hybrids with other Callithrix . This is the first study to evaluate sperm freezing from black‐tufted‐ear marmoset. Methods Testicles from all animals were measured, and semen was collected by penile vibrostimulation. Samples were analyzed after collection, after chilling and addition of glycerol, and after thawing. Results and Conclusions Fresh semen from both species was similar in many aspects. Additionally, there is a relationship between total motility, plasma membrane integrity, and acrosome integrity observed in sperm samples from both species. We managed to evaluate fresh and thawed sperm with suitable methods for use under zoo or field conditions. However, TEST egg yolk with glycerol at 4% and 6% concentration was not effective for sperm protection in both species during cryopreservation.